An Unexpected Experimental Pitfall in the Molecular Diagnosis of Bacterial Endophthalmitis
نویسندگان
چکیده
منابع مشابه
An unexpected experimental pitfall in the molecular diagnosis of bacterial endophthalmitis.
General primer-mediated ribosomal DNA amplification during endophthalmitis may improve the quality of diagnostic microbiology. However, extreme care needs to be taken not to introduce contaminating bacterial DNA during surgery procedures. The use of decontaminating iodine solutions can lead to such contamination due to the presence of DNA from Pseudomonas-like organisms.
متن کاملMolecular biology applied to the laboratory diagnosis of bacterial endophthalmitis.
Bacterial endophthalmitis is a serious but uncommon intraocular infection which frequently results in vision loss. Early diagnosis and appropriate therapy are associated with better visual outcome. Conventional microbiological methods are currently used for microbiological characterization of eyes with suspected endophthalmitis. However, the sensitivity of bacterial detection from aqueous and v...
متن کاملPolymerase chain reaction in the diagnosis of bacterial endophthalmitis.
BACKGROUND Microbiological investigations of vitreous fluid (VF) and aqueous humour (AH) specimens have often failed to detect the infecting agent in infectious endophthalmitis, resulting in a clinical dilemma regarding therapy. In this study, the polymerase chain reaction (PCR) was evaluated in the diagnosis of bacterial and Propionibacterium acnes endophthalmitis. METHODS 58 intraocular spe...
متن کاملSpontaneous inhibition of bacterial growth in experimental gram-negative endophthalmitis.
We compared the growth patterns of gram-negative bacilli in the vitreous humor (experimental endophthalmitis in rabbits) and in a subcutaneous site of infection (croton oil pouches in rats). In untreated animals, inoculation of Klebsiella pneumoniae or Pseudomonas aeruginosa into either site was followed by a period of rapid bacterial multiplication. Thereafter, the numbers of bacteria in the v...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2004
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.42.11.5403-5405.2004